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KMID : 0376219860230020333
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Chonnam Medical Journal
1986 Volume.23 No. 2 p.333 ~ p.340
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Ferrous Ion-Induced Oxidation of Hemoglobin
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李民化/Lee, Min-wha
梁誠烈/朴昞柱/姜亨求/Yang, Sung-yeul/Park, Byung-ju/Kang, Heong-koo
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Abstract
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Ferrous ion at low concentrations has been shown to catalyze the oxidation of oxyhemoglobin to methemoglobin at pH 5.0. The initial oxidation of hemoglobin proceeded in proportion to the concentrations of Fe2+ (0.4 mM to 4.0 mM) and- slowed down in later stages until it reached the maximal 80% oxidation. The oxidation was enhanced in the presence of cheating agents such as ethylenediamine tetracetate, oxalate and adenosine diphosphate, but decreased rather in the presence of citrate. Although the Fe2+-induced oxidation of hemoglobin at later stages was slightly enhanced by superoxide dismutase, it was markedly inhibited by catalase. Hydroxyl radical scavengers such as thiourea and benzoate had no effect on the hemoglobin oxidation by Fe2+, but they exerted an inhibitory effect on the oxidation of hemoglobin caused by Fenton´s reagent (Fe2+, H2O2).
Based on these results, it is proposed that hemoglobin is oxidized by superoxide radical and hydrogen peroxide which are formed from the autoxidation of Fe+2, and that the participation of free hydroxyl radical in this process is minimal.
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